With a unique combination of innovative buffers, genetically engineered, high-performance Taq DNA polymerase and superior hot-start technology, Invitrogen Platinum II Taq Hot Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR.

Platinum II Taq Hot Start DNA Polymerase features include:
•Innovative Buffer—achieves universal annealing temperatures by stabilizing the primer-template duplex
•Engineered Taq DNA Polymerase—enables rapid cycling and is tolerant to commonly used inhibitors
•Platinum Hot Start Technology—provides superior specificity, sensitivity, and Yield; supports room temperature reaction setup

Platinum II Taq Hot Start DNA Polymerase is an engineered Taq DNA polymerase that is more resistant to reaction inhibitors generated by sample material or DNA purification steps tolerance. The enzyme has a faster DNA synthesis rate and PCR results are typically obtained more than twice as fast as with other Taq DNA polymerases. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperature and dissociate after an initial denaturation step at 94°C. This automated “hot start” provides increased sensitivity, specificity, and yield while also enabling reaction assembly at room temperature.

Due to the unique composition of Platinum II PCR buffer, the annealing temperature is 60°C for most primer pairs designed according to general design rules. Co-stabilizing components in the buffer can enhance the stability of the primer-template complex during the annealing process and achieve higher specificity without the need to optimize the annealing temperature for each primer pair. Using Platinum II Taq Hot Start DNA Polymerase, different PCR assays can be cycled simultaneously using the same protocol using universal primer annealing temperatures and extension steps selected for the longest fragment to be amplified.

Platinum II Taq Hot Start DNA Polymerase has an optional Platinum GC Enhancer for specific amplification and increased yield of high GC content targets.

Use Platinum II Taq Hot Start DNA Polymerase for amplification of DNA from complex genomic, viral and plasmid templates and for RT-PCR, genotyping, high-throughput PCR or applications where sample purity is suboptimal .

For added convenience, we also offer Platinum II Taq Hot Start PCR Master Mix (2X) , which contains Platinum II Taq Hot-Start DNA Polymerase (supplied as a ready-to-use mix) along with Platinum II PCR Buffer and dNTPs to reduce pipetting steps during PCR reaction preparation. We also offer Platinum II Hot Start Green PCR Master Mix (2X), which additionally contains density reagent and two A tracking dye allows direct loading of PCR products onto the gel, further simplifying the PCR workflow from preparation to result analysis.

For more information, please visit www.thermofisher.cn/platinumiitaq›