Basic Eagle Medium (BME) is a widely used synthetic basal medium that supports many types of mammalian cells grow. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, who discovered the minimum requirements for cell growth in vitro. BME has since been used in other human cell lines, including WI-38 and MRC-5.
Provides complete Formulation.

BME contains eight B vitamins, ten essential amino acids, as well as cystine, tyrosine and glutamine. Several modifications of BME have been made: minimum essential medium, Glasgow's medium, and Dulbecco's modified Eagle's medium. This BME formula contains Earle's salt and is suitable for use in CO₂ incubators.

Intended Use of Product
For in vitro diagnostic use. Note: Not for therapeutic use in humans or animals. Use other than for product labeling purposes may violate local regulations.

Dual-site cGMP manufacturing
Gibco™ BME is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure supply chain stability, we also offer the same Gibco™ BME product (410100-026) manufactured in our Scottish factory. The facility is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.

BME does not contain proteins, lipids or growth factors. Therefore, BME needs to be supplemented with nutrients, usually 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.