Use this kit to obtain up to 5 log enrichment of human epithelial tumor cells directly from whole blood, bone marrow or PBMC . Tumor cell clumps can be enriched from bone marrow. Enriched cells are bead-free, viable and suitable for staining with immunocytochemistry techniques or any other downstream application.
Highly enriched in living human tumor cells
• Isolated directly from whole blood, bone marrow or PBMC
• Live cells for any cell application
Method: Add CELLection™ Dynabeads™ to the sample. After a short incubation period, the beads will bind to the tumor cells. Tumor cells bound to the beads are rapidly separated on a magnetic stand and subsequently released from the beads using the provided release buffer. (CELLection™ Dynabeads™ contain a DNA linker that is cleaved by DNase I in release buffer.)
Note: If you wish to remove or use isolated tumors For cells used in molecular applications, use the equivalent product Dynabeads™ Epithelial Enrichment (Cat. No. 161-02). This product contains the same antibodies but does not release cells from the beads after positive isolation.
Starting samples: Whole blood, bone marrow, buffy coat, PBMC.
For Research Use Only. Not for use in diagnostic procedures.