Thermo Scientific GeneJET Plant Genomic DNA Purification Miniprep Kit is designed for fast and efficient purification from a variety of plant species and tissue types Designed with high quality genomic DNA. The kit uses silica membrane technology in a convenient spin column format, eliminating the need for expensive resins, toxic phenol-chloroform extraction, or time-consuming alcohol precipitation.

Product Advantages

•Fast—The procedure is completed within 30 minutes after cell lysis
•Convenient—Protocol does not include clogging-prone lysate filtration step
•High Yield—Get up to 32 μg of high-quality DNA (including nuclear, mitochondrial, and chloroplast DNA) from 100 mg sample< br>•Compatible—Suitable for a variety of samples (leaves, roots, buds, leaves and other parts of various plants)

After cell lysis, the standard process takes less than 30 minutes Complete, the resulting purified DNA is larger than 30 kb. DNA yield varies between species and tissues depending on genome size, ploidy, cell number, and age of the tissue sample. Typical yields from standard tissue sources (eg, young wheat leaves) are 30 to 32 μg from 100 mg of tissue. The isolated DNA can be directly used in PCR, qPCR, Southern blotting and enzymatic reactions.

Applications

•Isolated genomic DNA is ideal for routine molecular biology procedures, including:
•PCR and qPCR
• FastDigest and Conventional restrictiondigestion
• Southern blot