DNase I (deoxyribonuclease I) cleaves single- and double-stranded DNA into oligodeoxygens containing 5' phosphates Ribonucleotides. RNase is reduced to undetectable levels.

Applications
DNase I is suitable for removing DNA from protein preparations, nick-translating DNA, and generating random fragments for dideoxy sequencing.

Note: To remove DNA from RNA preparations, use amplification-grade DNase I (Cat. No. 18068-015).

Source
DNase I is purified from bovine pancreas.

Specific Activity
The specific activity of DNase I is typically in the range of 10,000–25,000 units/mg.