Thermo Scientific Pierce BS3-d0/d4 reagents form a matched set of heavy (deuterated) and light BS3 amine reactions Sexual cross-linking agents that aid in the identification of cross-linked peptides by mass spectrometry.

BS3-d0/d4 reagent is a commonly used BS3 cross-linking agent (Product No. 21580 ) quality analogues. d-0 (BS3-d0) has ordinary hydrogen atoms, while d-4 (BS3-d4) contains four deuterium atoms, making it weigh exactly four hydrogen mass units. When the two types are mixed and used to cross-link interacting proteins, a special mass pattern is produced that is able to unambiguously identify the cross-linked molecules.

BS3-d0d4 Reagent Features:

•Deuterium-labeled BS3 and its specially produced standard pure hydrogen analog for cross-linking protein structure:functional analysis by mass spectrometry —
• Easy identification of cross-linked fragments—A 4-unit difference in peptide mass unambiguously identifies cross-links at specific loci in protein interaction structures
• < b>Application Specific Instructions—Help first-time users understand the application
• Method requires only micrograms of protein—For NMR or X-ray crystallography that requires large amounts of protein, special solvents, or crystal formation chemical method and is an excellent alternative

The addition of equimolar mixtures of two identical cross-linkers differing only in the number of deuterium atoms in their chemical structure provides a means of identifying low-abundance single cross-linkers by mass spectrometry An effective method of linking peptides. The interaction of cross-linked proteins with the BS2G-d0/d4 reagent produces a special mass pattern in which the cross-linked peptides differ by 4 mass units after digestion of the cross-linked protein or protein complex. Further analysis of the reaction products can yield low-resolution three-dimensional structural information.

BS3-d0/d4 and BS2G-d0/BS2G-d4 Reagent is a homobifunctional, amine reactive, non-cleavable, water-soluble cross-linking agent with specified Spacer arm length. These reagents can be used as molecular rulers for estimation of spatial relationships in protein structure-function studies. Both light and deuterated analogues react efficiently with primary amines (—NH2) at pH 7-9 to form stable amide bonds. Proteins typically contain lysine (K) residue side chains and several primary amines in the N-terminus of each polypeptide, which serve as targets for the two sulfo-NHS ester reactive groups found in these reagents. These reagents are supplied as sodium salts and are soluble in water at concentrations up to 10 mM.

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BS3-d0 (bis(sulfo) Succinimide) suberic acid-d0)