Thermo Scientific Pierce SAT(PEG)4 is a long-arm (18.2 Angstrom), pegylated form of SATA. It is a sulfhydryl addition reagent used for the covalent modification of primary amines to facilitate controlled heterobifunctional cross-linking.

Features of SAT(PEG)4:

• Complete thiolation and PEGylation of primary amines in one step
• Add sulfhydryl groups for heterobifunctional cross-linking without affecting the protein Solubility
•NHS activation for efficient PEGylation of primary amines at pH 7-9; reaction of NHS ester groups forms stable, irreversible amide bonds
•Has defined structure and molecular weight of pure compounds, ensuring reproducible protein modification effects
•PEG spacers offer unique advantages, including increased stability, reduced aggregation tendency and reduced immunogenicity

SAT(PEG)4 isN-Succinimide S-acetyl (thiotetraethylene glycol) is a reactive amine used for pegylation and sulfhydryl addition to proteins and other amine-containing molecules. Reagents. Similar to SATA and other S-acetyl (SAT) reagents, SAT(PEG)4 is most commonly used Bioconjugation methods to incorporate sulfhydryl (—SH) groups into proteins as part of a two-step or heterobifunctional cross-linking strategy. The SAT group protects the sulfur atom from oxidation, but when an unprotected sulfhydryl group is required for coupling, hydroxylamine can be used Process it to remove it easily. The polyethylene glycol (PEG) spacer arm in SAT(PEG)4 minimizes steric hindrance and improves the solubility of modified and coupled molecules.

Specific reactivity towards primary amines, such as lysine residues in proteins, can be achieved through NHS ester groups. Spontaneous reactions in phosphate or other non-amine buffers at pH 7-9 form stable covalent amide bonds. When using hydroxylamine for sulfhydryl deprotection, the SAT(PEG)4-modified molecule can be combined with other proteins or Surface (passed by Sulfo-SMCC or SM(PEG)n reagent and other heterogeneous bifunctional cross-linking agents realize maleimide activation) reaction.

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