TaqMan Fast Universal Master Mix in 20-μL reaction volumes using Applied Biosystems 7900HT and Fast 7500 Fast Real-Time PCR Systems (2X) (without AmpErase UNG) provides results from 40 PCR cycles in 40 minutes. The optimized formulation contains AmpliTaq Fast DNA Polymerase UP, a highly purified DNA polymerase designed to allow rapid hot-start, minimize the formation of non-specific products, and enable room temperature reaction setup. In addition, a proprietary ROX dye acts as a passive internal control to normalize non-PCR-related fluorescence fluctuations, enabling extremely high precision on Applied Biosystems real-time PCR instruments.

Convenient
Contains everything needed for TaqMan-based qPCR amplification and detection in a single tube. TaqMan Fast Universal PCR Master Mix contains all the components except template and primers for superior real-time qPCR.

Compatibility
TaqMan Fast Universal PCR Master Mix (2X) (without AmpErase UNG) is compatible with TaqMan Gene Expression Assays.

Alternative products: Try TaqMan Fast Advanced Master Mix , our higher performance probe master mix. With TaqMan Fast Advanced Master Mix, we've been able to take full advantage of TaqMan Fast Universal PCR Master Mix and add more functionality to your gene expression analysis.