High Capacity RNA-to-cDNA™ Kit is an improved reverse transcription kit, when combined with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix and other PCR enzymes provide excellent performance when used together.

Features of this kit include:
Fast – short reaction time (typically 30–60 minutes)
• Convenience – Simple protocol with fewer pipetting steps
• Reliable – can be used on both high and low abundance targets
• Flexible – optimized The two-step experimental protocol can realize multiple PCR reactions in a single reverse transcription reaction

Simplify cDNA synthesis while maintaining high sensitivity of detection
This double tube The components of the kit work together to perform reverse transcription with high sensitivity and specificity for a wide range of template amounts. When serially diluted RNA concentration reference standards were amplified, excellent reverse transcription and PCR efficiencies were demonstrated over 11 orders of magnitude. This wide dynamic range can support different reaction conditions to detect transcription from highly active to low-expressed genes. The dual-tube master mix format reduces reaction time and requires fewer pipetting steps than the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.