RNaseAlert™ QC System v2 detects RNase activity with a convenient and sensitive fluorometric assay that provides real-time results. This version contains a substrate that is more sensitive (∼60%) than the previous version. This kit contains enough reagents for 5 x 96 (480) high-throughput assays.

The RnaseAlert™ QC System v2 features:

• Detection of RNase A as low as ∼0.3 pg using a fluorometer
• Simple and straightforward 30-minute assay
• Design For monitoring RNase activity in column fractions during protein purification
• Use with the DNaseAlert™ QC System to quantitatively detect RNase and DNase in the same sample

RNaseAlert™ QC System v2 features a new and sensitive A new type of higher RNA substrate, which is labeled with a fluorescent reporter molecule (fluor) on one end and a quenching group on the other end. In the absence of RNase, the physical proximity of the quencher suppresses the fluorescence in the fluor to very low levels. However, when RNase is present, the RNA substrate is cleaved and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength.

Fluorescence can be easily detected with filter or monochromator based fluorometers. Because the fluorescence of RNaseAlert™ substrate gradually increases in the presence of RNase activity, results obtained using fluorometer monitoring can be evaluated kinetically. The sequence of RNaseAlert™ Substrate has been carefully optimized to detect several RNases, including RNase A, RNase T1, RNase I, Micrococcal Nuclease, S1 Nuclease, Mung Bean Nuclease, and Benzonase™.

Fast and Convenient Protocols
The RNaseAlert™ program takes only minutes to set up. Lyophilized fluorogenic substrate is reconstituted in the provided tube and incubated with detection solution for several minutes. The results are then read using a fluorometer. Samples that fluoresce significantly compared to negative controls are contaminated and should not be used with RNA. Nuclease-free water and RNase A are provided as controls.

Accessory Products
RNaseAlert™ QC System v2 and DNaseAlert™ QC System Fully compatible. Because DNaseAlert™ Substrate contains a fluorescent label that is spectrally different from RNaseAlert™ Substrate, both kits can be used simultaneously for quantitative real-time analysis of RNases and DNases in a single sample.