Gateway™ Entry Vectors are designed to clone DNA sequences using restriction enzymes and ligases to create Gateway™ Entry clones. The resulting entry clone is ready for recombination with a destination vector to create an expression clone.

Gateway™ Entry Vectors (Table 1) provide the following:
• attL1 and attL2 sites for site-specific recombination of entry clones with the Gateway™ Destination Vector to ensure appropriate Clone genes of interest in the direction of expression
• Kozak consensus sequence for efficient translation initiation in eukaryotic systems
• Ribosome binding sites for efficient translation initiation in prokaryotic systems (pENTR™ only 1A Dual Selection, pENTR™3C Dual Selection and pENTR™11 Dual Selection Vectors)
• rrnB transcription termination sequence, used to prevent basal expression of the associated PCR product in E. coli
• pUC replication region sequence, used Plasmid for high copy replication and maintenance in E. coli
• Kanamycin resistance gene for selection in E. coli
• ccdB⁄ chloramphenicol fusion gene located between two attL sites , for
o negative selection and for selection of chloramphenicol in
o E. coli
• Kanamycin resistance gene, for selection in E. coli