Total exosome isolation reagent (from other body fluids) can be obtained from cerebrospinal fluid (CSF) following a protocol that scales with sample size ), ascites, amniotic fluid, breast milk and saliva to quickly and efficiently enrich intact exosomes.

• Dramatically improve recovery of intact exosomes for any type of downstream application
• Easily isolate exosomes following a simple and reliable protocol
• Avoid time-consuming ultracentrifugation
• Maximize flexibility—can be scaled up or down based on sample volume

Total Exosome Isolation Reagent binds water molecules, forcing poorly soluble components (such as vesicles) out of solution and subsequently These poorly soluble components can be collected through a short period of low-speed centrifugation. The reagent is added to the body fluid and the solution is incubated at 2–8°C or room temperature (temperature and incubation time depend on the specific body fluid). Pelleted exosomes were recovered by standard centrifugation (speed: 10,000 xg). The pellets are then resuspended in PBS or similar buffer, at which point the exosomes are ready for downstream analysis or further purified by affinity methods. Total RNA and protein can be purified using the Total Exosome RNA and Protein Isolation Kit (Cat. No. 4478545), or intact exosomes can be used for biological studies involving their pathways, function, and transport.

For downstream mass spectrometry (MS) analysis, we recommend gel electrophoresis purification of the isolated exosome suspension prior to analysis.