Thermo Fisher 60110-104 Each SMART Digestimmunoaffinity (IA)Streptavidin kit
$2356.29 / Parcel$2583.66 Min.order:1
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Achieve fast, simple, highly reproducible, and sensitive immunocapture and protein digestion in a single well using the Thermo Scientific™ SMART Digest™ Immunoaffinity (IA) Streptavidin Kit. These kits are designed to eliminate challenges often encountered during biomarker quantitation by providing an easy-to-use capture digestion workflow that takes only a few hours, not overnight. This kit purifies a variety of target biomolecules by forming strong streptavidin-biotin bonds.

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Meeting the Challenges of Low-Level Quantitation in Complex Matrices

The need to improve the ability to identify low-level biomarkers has forced the analytical industry to face extremely high levels of analyte and sample complexity, making quantitation a challenging endeavor.

Since these proteins are often present at low levels in complex biological matrices, an immunoaffinity capture step is often used to purify the sample and increase sensitivity. This step is often followed by protein digestion. Current processes are often time-consuming, multi-step, and prone to non-reproducibility.

Immunocapture and protein digestion in a single well

These IA kits offer a process that is:

  • Fast
  • Simple
  • Highly reproducible
  • Sensitive
Unique design reduces workflow to hours

In the SMART Digest IA kit, the immunocapture reagent and temperature-activated, thermostable trypsin are co-immobilized on the resin. Following enrichment, digestion is accelerated by high temperature activation of the enzyme under protein denaturing conditions, resulting in a workflow that takes hours instead of overnight.