The need to identify low-level biomarkers forces the analytical industry to face extremely high levels of analyte and sample complexity, making quantitative analysis a challenging task. Challenging work.

Because these proteins are often present at low levels in complex biological matrices, immunoaffinity capture steps are often used to purify samples and increase sensitivity. This IA step is usually followed by protein digestion. Current processes are often time-consuming, involve multiple steps, and are easily non-reproducible. The SMART Digest IA kit solves these problems.

These IA kits provide a process that is:

• Fast< /li>
• Simple
• Highly repeatable
• Sensitive

In the SMART Digest IA Kit, the immunocapture reagent and temperature-activated thermostable trypsin are co-immobilized on the resin. After enrichment, digestion is accelerated by activating enzymes at high temperature under protein denaturing conditions, allowing the workflow to take hours instead of overnight.