This gentle and easy-to-use kit allows you to isolate flow cytometry-compatible DNA from PBMCs or directly from whole blood. of human CD8+ T cells. The magnetic beads are released and removed from the cell sample immediately after positive isolation. In this way, you avoid exposing cells to potentially cytotoxic or immunogenic foreign substances, such as iron oxides or dextran, which are potential issues when using biodegradable particles. Additionally, your cells will not be stressed by passing through the column. You can expect to obtain purity levels of >95% human CD8+ cells.

Advantages:

• No risk of results being affected by separation method
• Gentle test tube method - no columns required
• High purity and recovery
• Magnetic bead-free cells - compatible with flow cytometry analysis
• Compatible with flow cytometry analysis
• Magnetic bead-free live CD8+ T cells that can be used directly in any cell-based assay .

The isolated cells were presumed to have expression profiles of memory and naïve CD8+ T cells and showed high proliferative capacity. You'll save time and money and be more confident in your results.

Starting sample:
MNC or whole blood.

Capacity:
Kit will process 2 ×10⁹ mononuclear cells (MNC) or 80 mL of whole blood