Thermo Scientific Pierce Hoechst 33342 fluorescent dye is a high-quality Hoechst dye solution that can be used for DNA and cell nuclei in cell imaging technology. Fluorescent staining of fixed and live cells.

Hoechst 33342 Fluorescent Dye Features:

•Hoechst Dye—a blue fluorescent dye specifically for DNA, the nucleus of eukaryotic cells
• Convenience—Available in an easy-to-use Hoechst dye solution (20 mM)
• Cell Imaging—Cell-penetrating dye is effective for staining fixed and live cells
• Counterstain—For use with fluorescence microscopy or target-specific detection with high content screening (HCS) fluorescent antibodies

Hoechst 33342 (2'-[4 -Ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5'-bi-1H-benzimidazole trihydrochloride trihydrate) is a cell-permeable DNA stain agent that can be excited by UV light and emits blue fluorescence at 460 to 490 nm. Hoechst 33342 preferentially binds to the adenine-thymine (AT) region of DNA. The dye binds to the minor groove of DNA and exhibits different fluorescence emission spectra depending on the dye:base ratio.

Characteristics of Hoechst fluorescent dyes
As counterstains for fluorescence imaging methods, Hoechst dyes are compatiblefluorescein and rhodamine dye-labeled antibodies and Other probes as well as Thermo Scientific DyLight Fluor. Stable 20 mM aqueous stock solutions are basically ready for use.

Hoechst33342 For specific staining of nuclei in living or fixed cells and tissues. This stain is often used in combination with the 5-bromo-2'-deoxyuridine (BrdU) label to differentiate the compact chromatin of apoptotic nuclei, thereby identifying replicating cells and classifying cells based on cellular DNA content. Hoechst 33342 and propidium iodide are often used together for simultaneous flow cytometry and fluorescence imaging analysis of various stages of apoptosis and cell cycle distribution.