Use modified nucleotide ethynyluridine (EU, E10345) or use Click-iT nascent RNA capture reagent Cassette (C-10365) detects temporal and spatial changes in total RNA transcription in vitro and in vivo.
Both detection methods use powerful click chemistry, a simple and reliable two-step technology. In the first step, the cell or animal is fed with an alkyne-containing biomolecule and actively incorporated into the RNA. The assay utilizes a chemoselective linkage or "click" reaction between an azide and an alkyne, using the corresponding azide-containing dye or hapten with the Click-iT™ Cell Reaction Buffer Kit to detect modified proteins . Cellular analysis can be performed using fluorescence microscopy, flow cytometry, or high-content imaging and analysis (HCS).
By using the Click-iT nascent RNA capture kit, nascent RNA can be isolated for transcriptome analysis, including RNAseq, ΔCT, RNA turnover, nuclear contiguous and runaway transcription, array analysis, etc. Please view this link for more details on the Click-iT Nascent RNA Capture Kit: Click here.