RPMI 1640 medium (Roswell Park Memorial Institute 1640 medium) was originally developed for suspension culture of human leukemia cell monolayers . RPMI 1640 was found to be effective on a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and cancer cells. We offer a variety of RPMI 1640 modifications for a wide range of cell culture applications. Use Medium Selection Tool Find the right recipe.
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Use RPMI 1640 medium
RPMI 1640 medium is unique compared to other media because it contains the reducing agent glutathione and high concentrations of vitamins . RPMI 1640 medium contains biotin, vitamin B₁₂ and PABA, which are not found in Eagle's Minimum Essential Medium or Dulbecco's Modified Eagle's Medium. In addition, the medium contains high concentrations of the vitamins inositol and choline. Contains GlutaMAX Supplemented RPMI 1640 Medium™ minimizes the build-up of toxic ammonia and improves cell viability and growth (easy-to-use format). RPMI 1640 medium does not contain proteins, fats or growth factors. Therefore, RPMI 1640 medium requires supplements, usually 10% Fetal Bovine Serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

cGMP Manufacturing and Quality System
RPMI 1640 media is manufactured in a cGMP compliant facility in Grand Island, New York. The facility is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure a stable supply chain, we offer the same RPMI 1640 product (72400-054) manufactured in our factory in Scotland. The factory is also an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.