DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium , can be used to support the growth of many different types of mammalian cells. Cells that have been successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We provide a variety of DMEM/F-12 modified media for a wide range of cell culture applications. Use Medium Selection Tool Find the right recipe.
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Use DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. The formula contains DMEM's high concentrations of glucose, amino acids, vitamins and various components of F-12. Contains GlutaMAX™ supplemented DMEM/F-12 in an easy-to-use packaged form significantly reduces the buildup of toxic ammonia and improves cell viability and growth. DMEM/F-12 contains no proteins, lipids or growth factors. Therefore, DMEM/F-12 may require nutritional supplementation, typically 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires an environment of 5–10% CO₂ to maintain physiological pH.

cGMP Manufacturing and Quality Systems
DMEM/F-12 is manufactured in a cGMP compliant facility in Grand Island, New York. The factory is an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard. To ensure a stable supply chain, we also offer the same DMEM/F-12 product (31331-028) manufactured in our Scottish factory. The factory is also an FDA-registered medical device manufacturer and is certified to the ISO 13485 standard.