Thermo Scientific Bond-Breaker TCEP Solution (neutral pH) is a stable version of thiol-free phosphine-containing TCEP compounds A 0.5M solution that can be used as a 10X stock solution for addition to SDS-PAGE sample loading buffer to reduce protein disulfide bonds.

Bond-Breaker TCEP Solution (Neutral pH) Features:

•Odorless—Unlike DTT and β-ME, TCEP is odorless and helps For a healthier laboratory environment
•Efficient—5 to 50 mM TCEP completely reduces most peptide or protein disulfide bonds in minutes (i.e., as efficient as DTT)< br>•Specific—selective and complete reduction of even extremely stable water-soluble alkyl disulfides
•Fast—at room temperature and pH Protein disulfides can be reduced within five minutes under conditions of 5
•Stable—resistant to air oxidation; non-volatile and does not react with other functional groups found in proteins
•Universal—reduces peptides and proteins over a wide range of pH, salt, detergent, and temperature conditions
•Compatible—in most applications (e.g., There is no need to remove the reducing agent before purification of histidine-tagged proteins, maleimide coupling) because TCEP does not contain sulfhydryl groups

Bond-Breaker TCEP solution is a potent, odorless, thiol-free solution Reducing agent widely used in protein and other studies involving the reduction of disulfide bonds. This product is an efficient and convenient alternative to SDS-PAGE sample buffer containing B-mercaptoethanol or DTT. The neutral pH of this reagent provides clear bands and avoids exposure of proteins to the strong acid of TCEP·HCl, which can cause hydrolysis and carbohydrate modification.

Notes on using Bond-Breaker TCEP Solution:
• Available in a wide range of pH values (pH 4.0-9.0) and temperatures (5 to 95°C) Perform a restore.
• Efficiently reduce most proteins without the use of denaturants. However, the addition of denaturants such as guanidine hydrochloride may help expose internal disulfides to immobilized TCEP. ·
•Urea is not recommended as a denaturant because it forms cyanates that react with sulfhydryl groups.
•Do not expose metal to TCEP solution as this will reduce TCEP activity.
•During the reduction process, adding 5 to 20 mM EDTA to the sample buffer helps prevent reoxidation of sulfhydryl groups by divalent metals such as Zn 2+, Cu 2+, and Mg 2+.
•Reduced samples should be used immediately after reduction because disulfides will reform over time.

Related products
Pierce™ TCEP-HCl
Pierce™ DTT (dithiothreitol)