Thermo Scientific M-PER Mammalian Protein Extraction Reagent is used to efficiently extract total soluble protein from cultured mammalian cells. .

Features of M-PER Mammalian Protein Extraction Reagent:

• Mild Action - Mild detergent cleavage, the resulting extract is directly used Compatible with Bradford (Bradford) protein quantitative analysis reagent or SDS-PAGE
• Strong compatibility—The extracted soluble protein is in a non-denatured state and can be directly used in immunoprecipitation reactions and other Affinity purification process
• Amine-free and dialyzable—ensuring compatibility with subsequent analysis systems
• Convenient—adherent cells directly on the plate Lyse on lysate or resuspend in solution after stripping and washing
• Native — Maintains activity of luciferase, beta-galactosidase, CAT and other reporters, with other suppliers The product is as good or better than the freeze/thaw method

This complete cell lysis reagent is a non-denaturing detergent formula that dissolves cell membranes and extracts total soluble cells in as little as 5 minutes protein. M-PER reagent requires little or no mechanical disruption and yields more protein than freeze/thaw cycle methods and sonication methods. This mammalian cell lysis reagent is so effective that it does not require detachment of adherent cells from the culture dish and can be simply and directly lysed and analyzed in 24- and 96-well plates of growing cells. The final lysates from both adherent and suspension cells are compatible with many downstream assays, including immunoassays, enzymatic assays, and a variety of common reporter gene assays.

Protein extraction is often a critical first step in any proteomic analysis process. First the cells must be lysed in order to open the cells and release the proteins of interest. Several methods commonly used to physically lyse cells include mechanical disruption, liquid homogenization, ultrasonic lysis, freeze/thaw cycles, and manual grinding.

Related resources:
Overview of cell lysis and protein extraction

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