Automated desalting and pH reduction in oligosaccharide separationsThe Dionex ERD 500 electrolytically regenerated desalter lowers sample pH and removes more than 99% of the salt by replacing sodium ions with hydronium ions. Sodium ions, thereby converting the sodium hydroxide and sodium acetate eluents into water and acetic acid. The volatile acetic acid makes it possible to produce lyophilized fractions without dialysis, making purified sugar samples available for further manipulations.
- Reduce the pH value of the eluate from ∼13 to 2-6
- No need for dialysis when freeze-drying the collected fractions, saving manpower
- From the fraction containing up to The eluent of 0.35 M Na+ removes more than 99% of sodium ions
- Maximize the dispersion of the sample flowing out of the detection cell and accurately collect the fractions
- Use electrolysis and trifluoroacetic acid ( TFA) regenerant to provide continuous desalter regeneration
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