Thermo Scientific B-PER Complete Bacterial Protein Extraction Reagent is an easy-to-use cell lysis reagent that contains nonionic A detergent solution that effectively destroys cells and solubilizes natural or recombinant proteins without denaturation. All B-PER reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA protein assay, Pierce 660 nm protein assay) . Depending on the specific application, protease inhibitors, salts, reducing agents, denaturants and chelating agents can be added to the reagents.

B-PER Bacterial Protein Extraction Reagent Features:

• Detergent in Tris buffer containing lysozyme and universal nuclease
• Improved cell lysis and DNA endonuclease, thereby releasing soluble proteins and reducing viscosity to increase yield
• Recovery of large molecular weight proteins (high molecular weight proteins)
• Cost-effectiveness equivalent to Merck Millipore Bugbuster™

B-PER Full Bacterial Protein Extraction Reagent is an all-inclusive formula that combines lysis reagents with lysozyme and universal nucleases to enable gentle extraction of Gram-negative and Gram-positive bacteria. Traditional enzyme-free Tris-buffered and phosphate-buffered formulations are also available. All B-PER reagents are compatible with downstream applications such as affinity chromatography (e.g., immobilized metal affinity chromatography, glutathione chromatography), SDS-PAGE, and protein quantification (e.g., Pierce BCA protein assay, Pierce 660 nm protein assay) . Depending on the specific application, protease inhibitors, salts, reducing agents, denaturants, and chelating agents can be added to the reagents.

All Thermo Scientific B-PER protein extraction reagents have the following features:
•Ready-to-use — formulated in Tris or phosphate buffer One-step cell lysis of Gram-positive and Gram-negative bacteria using mild non-ionic detergent (patented technology)
•Quick and easy — just add B- PER reagent is added to bacterial pellets, mixed and incubated for 10 to 15 minutes to recover soluble proteins after pelleting cell debris
•Convenience – B-PER Complete Set Reagents contain lysozyme and universal nuclease in a single formulation stored at 4°C
•Excellent yields — recover recombinant proteins from bacterial lysates or purify inclusion bodies to Near uniform levels
•Flexible — B-PER reagents are suitable for protein extraction at any ratio and are available in phosphate and 1X and 2X Tris formulations with or without enzyme
•< b>Compatible — Fully compatible with the addition of protease inhibitors, the resulting protein extract can be used for protein determination, typical affinity purification methods (such as GST, 6xHis) and other applications

Convenient ready-to-use format
B-PER bacterial extraction reagents are more efficient than traditional sonication and typical homemade lysis buffers, many of which contain detergents and can be used with downstream Apply interfering components. B-PER reagents are formulated in Tris or phosphate buffer at physiological pH. They extract native and soluble recombinant proteins and generate lysates that are directly compatible with most downstream workflows (such as electrophoresis, affinity purification, immunoprecipitation, protein interaction analysis, cross-linking and protein labeling).

More product data
•Improved all-in-one B-PER reagent, can be used for bacterial protein extraction

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B-PER™ Bacterial Protein Extraction Reagent
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