Thermo Scientific RIPA Lysis and Extraction Buffer is a high-quality, commonly used cell lysis reagent for cultured mammalian cells , ready-to-use and fully disclosed formulas.

RIPA Buffer Features:

•Convenience—Ready-to-use solution; no need to assemble and prepare components yourself
•Flexible< /b>— Compatible with many applications, including reporter gene detection, protein detection, immunoassay and protein purification
•Universal— Extracts cytoplasmic, membrane and nuclear proteins
•Disclosed Formula—Contains no proprietary components, giving the user full control and understanding of possible compatibility issues

The RIPA buffer can be obtained from cultured mammalian cells, including plated cells and Efficiently lyse and extract proteins from pellet suspension cells). This commonly used reagent extracts membrane, nuclear, and cytoplasmic proteins and is compatible with many applications, including reporter gene assays, Thermo Scientific BCA Protein Assay, Immunoassay and Protein Purification. Thermo Scientific Halt Protease Inhibitor Cocktail (Product No. 78430) and Halt Phosphatase Inhibitor Cocktail (Product No. 78420) and other inhibitors are also compatible with this RIPA buffer formulation and can be added before use to prevent Proteolyzes and maintains protein phosphorylation.

RIPA buffer takes its name from the original application for which it was developed: radioimmunoprecipitation assay. Although this isotope detection method is currently rarely used in laboratories, the acronym for this lysis buffer recipe has become widely used. RIPA Cell Lysis Reagent efficiently extracts proteins from a variety of cell types because it contains three non-ionic and ionic detergents. One disadvantage of this detergent formulation is that it is relatively incompatible with certain downstream applications compared to other lysis reagents.

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