pcDNA™ 3.4 TOPO™ vectors are constitutive mammalian expression vectors that provide ultra-high-level transgene expression. It can be used for transient protein expression in suspension-acclimated cells such as Expi293F™, FreeStyle™ 293-F and FreeStyle™ CHO-S cells. This vector can also be used as a Geneticin™-selectable expression plasmid to alter the genetic structure of stable cell lines. The enhanced full-length CMV promoter and other expression elements generally enable higher expression levels compared to pcDNA™ 3.1, 3.2 and 3.3-based expression constructs.

pcDNA 3.4 TOPO™ vectors contain the following elements:

•Full-length human cytomegalovirus (CMV) very early promoter/enhancer, capable of operating in a variety of Achieves high levels of gene expression in mammalian cells
• Downstream of the WPRE (Woodhog Post-transcriptional Regulatory Element) cloning site, which enhances transcriptional expression
• TOPO™ Clones sitecan quickly and efficiently (>85%) clone Taq-amplified PCR products
• Neomycin resistance gene is suitable for stable cell line screening with Geneticin™
• pUC replication region sequence can replicate and maintain plasmids in E. coli at high copies

pcDNA3.4™ TOPO™ TA Cloing Kit contains TOPO™ adaptive plasmid vector, Reagents for cloning selected genes into vectors via PCR, sequencing primers to confirm insertion direction, and One Shot™ TOP10 competent cells.