CorrectASE™ enzyme removes mismatches caused by oligonucleotide synthesis errors, allowing you to synthesize genes or fragments with Mutations reduced to 1/3 – 1/10 of the original size. By introducing CorrectASE™ enzymes into your own gene synthesis workflow, you can:

• Reduce the number of mutations in your synthetic genes or fragments
• By screening only each synthetic construct Reduce your labor time by sequencing only 2–4 clones instead of 10–16 clones
• Reduce your costs by sequencing only 2–4 clones instead of 10–16 synthetic genes< br>
Prevent unwanted mutations
During the synthesis process, commercially available synthetic oligonucleotides have a high error rate of every 300–1000 bases depending on the source. There is at least one error. These errors lead to frameshifts (deletions and insertions) and mismatch mutations during gene synthesis. Incubation with CorrectASE™ enzyme removes both mutation types.

After the initial PCR assembly of the oligonucleotides, an incubation step using CorrectASE™ enzyme is introduced. Denaturation and reannealing of the PCR product will result in all mutations being unmatched. CorrectASE™ enzyme binds to the resulting mismatch and creates a 3' nick in the erroneous DNA double strand. The enzyme's 3' to 5' exonuclease activity eliminates errors. A final PCR using a proofreading polymerase then assembles the correct fragments, increasing the likelihood that clones will be isolated with the correct sequence. Depending on the quality of the resulting oligonucleotides, only 2–4 clones need to be screened compared to 10–16 clones in a workflow that did not include the correction step. Add CorrectASE™ enzymes to your gene synthesis workflow to reduce labor time and sequencing costs.

Research use only. Not for therapeutic or diagnostic use in animals or humans.