The Molecular Probes™ ATP Assay Kit provides a convenient bioluminescent assay using recombinant firefly luciferase and its substrate D-luciferin to quantitatively determine ATP content. The assay kit is based on the absolute ATP requirements of luciferase for light production (maximum emission wavelength ∼560 nm at pH 7.8). The detection method is extremely sensitive; most chemiluminescence analyzers can detect as low as 0.1 pmol of pre-existing ATP or ATP formed in a kinetic reaction system. This sensitivity enables its use in a wide range of applications, including detection of ATP production in a variety of enzymatic reactions, including ATPase and NADPH oxidase, and detection of low levels in samples such as blood, milk, urine, soil, and sludge. Bacterial contamination. The luciferin–luciferase bioluminescence assay has also been used successfully to study the effects of antibiotics on bacterial populations, to determine cell proliferation and cytotoxicity in bacterial and mammalian cells, and to differentiate the effects of anticancer drugs on malignant cell growth. Inhibitory and cell killing potential.

ATP Content Assay Kit Specifications:
• Monitors luminescence using a chemiluminescence analyzer at ∼560 nm wavelength
• Can detect as little as 0.1 pmol of pre-existing ATP or ATP formed in a kinetic reaction system
• Luciferase and luciferin are individually packaged, allowing researchers to optimize conditions for their samples and instruments
• Contains sufficient reagents to use 500 µL Use a sample volume of 100 µL for 200 ATP determinations, or a sample volume of 100 µL for 1,000 ATP determinations

Find more cell viability and cytotoxicity assays as well as methods for detecting phosphate Probes for Metabolic Enzymes
For more information about these products, see the Molecular Probes™ brochureMultiple Cell Types Viability and Cytotoxicity Assay Kit - Section 15.3 and Detection of Phosphate and Polyphosphate Metabolizing Enzymes — Section 10.3.

For scientific research use only. Not for therapeutic or diagnostic use in humans or animals.