pVAX1 is specifically designed for the development of DNA vaccines. pVAX1 has the following properties:

• Eukaryotic DNA sequences are limited to those required for expression to minimize the possibility of chromosomal integration
• Reported in some individuals due to ampicillin Can produce allergic reactions, so the kanamycin resistance gene was used for E. coli screening
• The expression level of the recombinant protein from pVAX1 was the same as that obtained using its parental vector pcDNA™3.1 Quite

pVAX1's small size and multiple unique cloning sites simplify subcloning of even very large DNA fragments.