OxyBURST™ Green H₂DCFDA succinimidyl ester (2',7'-di Chlorofluorescein diacetate (SE) is an amine-reactive detection reagent that can be used to prepare oxidation-sensitive conjugates of a variety of biomolecules and particles, including antibodies, antigens, peptides, proteins, dextran, Bacteria, yeast and polystyrene microspheres, etc. After OxyBURST™ Green H₂DCFDA is coupled to an amine, it is treated with hydroxylamine at neutral pH to remove the two acetate esters to form a dihydrofluorescein conjugate. OxyBURST™ Green H₂ DCFDA conjugate is nonfluorescent, but upon oxidation forms the corresponding fluorescein derivative.

ROS indicator quality standards:
• Ex/Em: ∼492–495/517–527 nm
• Product is sensitive to air and should be stored in dry argon or nitrogen Medium
• The product can be dissolved in DMSO, DMF or ethanol for use
• The indicator is permeable to cells (see the literature for cell loading protocols)
• Flow cytometer, fluorometer, and microplate can be used Monitor the fluorescence signal with a reader or fluorescence microscope (equipped with an excitation light source and filters suitable for fluorescein)

Find more ROS indicators
We offer a range of Molecular Probes™ products for the generation of reactive oxygen species (ROS) (including singlet oxygen, superoxide, hydroxyl radicals, various peroxides and hydrogen peroxide) and for use in solutions Carry out corresponding fluorescence detection. For more information about these products, please consult the Molecular Probes™ HandbookSection 18.2—Generating and Detecting Reactive Oxygen.

For research use. Not for therapeutic or diagnostic use in humans or animals.