CellEvent™ Caspase-3/7 Green flow cytometry analysis kit can be used to detect apoptosis by flow cytometry. Activated caspase-3 and caspase-7 in apoptotic cells. The kit includes the novel fluorescent substrate CellEvent™ Caspase-3/7 Green detection reagent and SYTOX™ AADvanced™ dead cell stain.

•Caspase-3/7 specificity—includes recognition sequences for both caspase-3 and caspase-7
•Easy to identify—clearly identifies live, dead, and apoptotic cell populations
•Quick Analysis—no washing or fixation required
•Multicolor compatibility—conjugates other dyes susceptible to excitation by the 488 nm laser or other lasers

CellEvent™ Caspase-3/7 Green Detection Reagent is a cell Permeable reagent consisting of a four amino acid peptide (DEVD) coupled to a nucleic acid binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and cleave the caspase 3/7 recognition sequence encoding the DEVD polypeptide. Recognition of sequence breaks and binding of reagents to DNA marks apoptotic cells with a bright fluorescent signal (maximum absorption/emission wavelength ∼511/533 nm). When used with SYTOX™ AADvanced™ Dead Cell Stain, apoptotic cells can be easily differentiated from viable and necrotic cells.

Since no single parameter defines apoptosis in all systems, we strongly recommend using different combinations of measurements to reliably detect apoptosis.