BS(PEG)5 is a disuccinimidyl ester-activated PEG compound used in proteins and other molecules Cross-linking between primary amines (—NH2). The N-hydroxysuccinimide ester (NHS) groups at both ends of the PEG5 spacer react specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form a stable Amide bond.

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Features of BS(PEG)5:

•Reactive groups:Sulfo-NHS ester (both ends)
•Can react with the following groups: Amino group (primary amine)
• NHS ester reacts with —NH2 at pH 7-9 groups react to form stable amide bonds
• Water soluble; with non-PEGylated BS3 (also soluble ) and DSS (water-insoluble) are comparable
• Has membrane barrier Permeable for cell surface labeling
• Irreversibly cross-link proteins or peptides with flexible PEG spacers
• Polyethylene glycol spacers help maintain conjugate solubility
• Features Pure compounds with well-defined structure and molecular weight ensure reproducible protein modification effects
• PEG spacers offer unique advantages, including higher stability, lower tendency to accumulate, and lower immunogenicity
• Suitable for small molecule or peptide coupling

Other names for BS(PEG)n:

•Di-NHS-dPEG
•NHS-PEG-NHS
•BS( PEO)5
• Di(NHS)PEO5
• BS-PEO9
• dPEG NHS Ester
• Bis-succinimidyl ester PEG 400

Product Reference
Crosslinking Agent Application Guide -- Search the latest references for this product
Bioconjugate Techniques , Academic Press (Product No. 20036)

Related Products
BS(PEG)9 (PEGylated bis(sulfosuccinimide)suberic acid)
BS3 (bis(sulfosuccinimide) suberic acid)