The ATP-dependent oxidation of luciferin by luciferase produces light, so this system is used in plants, Reporters in bacterial and mammalian cells. Since chemiluminescence technology is virtually background-free, this reporter gene system is ideal for detecting low-level gene expression.

Molecular Probes™ Highly purified synthetic luciferin exhibits the same physical properties as natural luciferin. We offer fluorescein free acid (L2911) and its water-soluble sodium and potassium salts (L2912, L2916 ). DMNPE-masked D-luciferin (L7085) provides an efficient way to deliver fluorescein into intact cells. Methods can be used to provide a continuous source of active fluorescein. DMNPE-masked luciferin readily crosses cell membranes. Once inside the cell, luciferin can be released all at once by UV photolysis, or it can form active luciferin continuously and slowly by the action of intracellular esterases found in most eukaryotic cells.

Luciferase specifications:
•Chemiluminescence after oxidation of luciferase
•Luciferin potassium salt is easily soluble in aqueous buffer

Find more fluorescent and photoactivated probes
We offer a range of fluorescent substrates, photoactivated probes and masking probes. See Substrates for Microsomal Dealkylases, Acetyltransferases, Luciferases, and Other Enzymes—Section 10.6and< a href="http://www.invitrogen.cn/site/us/en/home/References/Molecular-Probes-The-Handbook/Crosslinking-and-Photoactivatable-Reagents/Photoactivatable-Reagents-Including-Photoreactive-Crosslinkers- and-Caged-Probes.html">Photoactive Reagents, Including Photoreactive Cross-Linkers and Masked Probes—Section 5.3 for more information on these products.

For research use. Not for therapeutic or diagnostic use in humans or animals.