pcDNA™ vectors enable high-level constitutive expression in a variety of mammalian cell lines. The pcDNA6.2/V5-DEST vector has the following main features:

• Cytomegalovirus (CMV) promoter can achieve high-level expression
•attR site In Gateway™ cloning, fragments flanked by attL can be used for recombination
•C-terminal V5 tag facilitates rapid detection
•Blasticidin resistance gene for high efficiency Stable Screening
•Ampicillin Resistance Gene and pUC Replication Region Sequences for E. coli Screening and Maintenance

Gateway™ Cloning
For all your expression needs, Invitrogen offers the advanced Gateway™ Destination vectors for expression in E. coli, insect, yeast or mammalian cells and for the production of native proteins or N- or C-terminal fusion proteins. Whether Entry or Ultimate™ RF clones, all Gateway™ destination vectors have anattR site for recombination using anyattL-side fragment. The table below lists a range of available destination vectors.

Additional materials required, available for purchase separately:Gateway™ Starter Clone, appropriate Gateway™ LR Clonase™ enzyme mix, and reaction buffer.