Thermo Scientific Pierce MS(PEG)24 is a methyl-terminated polyethylene glycol compound activated into NHS ester. For covalent PEGylation of proteins (e.g., lysine) or primary amines on the assay surface.

MS(PEG)24 Features:

•24 PEG units, 88.2Å spacer
•NHS activation for primary amines at pH 7-9 Efficient PEGylation; NHS-ester groups react to form stable, irreversible amide bonds
• Well-characterized PEGylation reagents with well-defined PEG chain lengths; molecules with discrete molecular weights to ensure protein modification applications Consistency of medium performance
•PEG spacers offer unique advantages, including increased stability, reduced tendency to aggregate, and reduced immunogenicity
•Easy-to-follow instructions increase likelihood of success

MS (PEG)n is an abbreviation for a group of compounds with a polyethylene glycol (PEG) spacer and methyl (—CH3) and amine-reactive NHS-ester groups at both ends. The unbranched, hydrophilic, discrete-length molecular form is methyl-PEGn-NHS ester, where the subscript 'n' indicates 4, 8, 12 or 24 ethylene glycol units. N-Hydroxysuccinimide (NHS) ester reacts spontaneously with primary amines (—NH2), providing efficient PEGylation of proteins, peptides, and other amine-containing molecules or surfaces.

PEGylation Applications:
•PEGylated amine surfaces
•Addition of inert materials to proteins, immunogens, drug compounds and probes
•Increase the solubility of proteins or peptides (reduce aggregation) without affecting functionality
•Protect proteins from proteolysis

Why PEGylate proteins or peptides?
PEG-containing reagents have been used to modify proteins to provide specific advantages. Protein PEGylation can improve the stability of the modified protein, prevent proteolytic enzyme cleavage, increase its half-life in biological applications, mask it to avoid immunogenic reactions, reduce its antigenicity or potential toxicity, and increase its solubility. Reduce the potential for accumulation and minimize interference in in vitro and in vivo applications. Polyethylene glycol, also known as polyethylene oxide (PEO), performs these functions because it is non-toxic, non-immunogenic, hydrophilic, water-soluble and highly flexible.

Advantages of Discrete Length mPEG-NHS Ester Compounds:
These reagents are specifically synthesized as homogeneous compounds with discrete chain lengths and well-defined molecular weights. Therefore, they enable precise control and optimization of surface protein modification experiments. In contrast, typical formulations of PEG compounds are heterogeneous mixtures consisting of a variety of chain lengths and a range of molecular weights.

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