Thermo Scientific Pierce High pH Reversed-Phase Peptide Separation Kit improves protein identification from LC/MS analysis through orthogonal peptide separation of complex peptide samples.

• Easy to use - Resin provided in single-use spin columns
• Improve protein identification - Protein identification increased by ≥50% compared to unseparated samples
• Reproducible - The difference in elution profile and component resolution is less than 20%
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• Optimized - Robust procedure that provides maximum protein identification and peptide recovery while minimizing component overlap
• Compatible - Passed Validated reagents for a variety of complex samples, including TMT™-labeled peptides

To achieve deep proteome sequencing, it is often necessary to reduce sample complexity through orthogonal dimensional separation prior to LC/MS analysis. The Pierce High pH Reversed Phase Peptide Separation Kit utilizes high pH reversed phase chromatography to separate peptides via hydrophobicity, providing excellent orthogonality to low pH reversed phase LC-MS gradients.
This kit is designed to improve protein identification through high pH separation protocols using patented reversed-phase resin in an easy-to-use spin column format. In contrast to strong cation exchange (SCX) separations, high pH reversed-phase fractions do not require additional desalting steps prior to LC/MS analysis.
High pH reversed-phase peptide separation kit includes high pH buffer (0.1% triethylamine) and twelve spin columns containing pH-resistant reversed-phase resin. Each reversed-phase separation spin column can separate 10-100 µg peptide samples in a microcentrifuge.
Native, phosphorylated, Tandem Mass Tag™ (TMT) labeled and other complex peptide mixture samples can be separated using this kit. Combining search results generated from single components helps improve protein sequence coverage and increases the number of identified proteins relative to unfractionated samples.
Applications:

• Reduce sample complexity to identify targets
• Conduct systems biology research
• Reduce sample complexity, To improve quantitative research