CytoVista Blocking Buffer is used when performing immunohistochemistry (IHC) or immunolabeling on tissue or 3D cell cultures. to block non-specific signals. The buffer consisted of PBS containing 0.2% Triton X-100, 6% donkey serum, and 10% DMSO. When used with other CytoVista products, it helps achieve clear, bright imaging at any tissue or 3D cell culture depth.

Learn more about CytoVista 3D Culture Clearing Reagents›

CytoVista Tissue and 3D Cell Culture Clearing Reagents Series features include:
• Rapid clearing of fluorescently labeled tissue for 3D imaging
• Minimized tissue shrinkage, swelling, or other morphological changes
• Simple workflow with no Requires no special instrumentation or equipment and can be easily adapted to IHC
• Compatible with most fluorophores, including dyes and fluorescent proteins

CytoVista tissue and 3D cell culture clearing workflow enables tissue and 3D cultured cells are cleared and minimized changes in morphology or detection sensitivity of nearly all fluorophores. Using this easy-to-use protocol, entire mouse brains (8 mm sections) can be cleared in 24 hours, or 1 mm sections can be cleared in 2 hours, without the use of any special instruments or equipment.

The CytoVista clearing workflow is compatible with most fluorophores, including fluorescent proteins detected using common fluorescence imaging instruments such as widefield microscopy, high content analyzers, confocal microscopy, and light sheet microscopy. For first-time users, we recommend the CytoVista Tissue Clearing/Staining Kit (Cat. No. V11324) or the CytoVista 3D Cell Culture Clearing/Staining Kit (Cat. No. V11325). These kits contain all necessary reagents for clearing and IHC staining.

CytoVista clearing technology effectively clears tissue for 3D fluorescence imaging, but unlike other clearing technologies, it does not significantly alter tissue morphology. Minimization of morphological changes such as shrinkage and shrinkage has been observed. For precious samples, clearing is reversible and the tissue can be processed for histological studies such as H&E staining.

For tissues that are easy to clear (such as brain, lung, intestine, skin) and tissues with a thickness less than 250 μm, processing with CytoVista Tissue Clearing Reagent is sufficient for 3D imaging. However, tissues that are thicker than 250 μm or that are difficult to clear (such as kidney, liver, heart, and placenta) need to be processed first with CytoVista Tissue Clearing Reagent and then with CytoVista Tissue Clearing Enhancer.

CytoVista 3D Cell Culture Clearing Reagent is recommended for spheroids or organoids up to 1 mm thick.

CytoVista tissue clearing reagent is not recommended for hard tissues such as bone, cartilage, and connective tissue.