CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent activates Caspase-3/7 Fluorescent indicator that requires no washing and is suitable for both live and fixed cell applications. Activation of caspase-3 is an early indicator of apoptosis, and CellEvent™ Caspase-3/7 Green Reagent provides rapid and sensitive detection of apoptotic cells.

• Simple, fast, no-wash protocol helps preserve fragile apoptotic cells
• Compatible with live cell fluorescence imaging and formaldehyde fixation methods
• Ready-to-use liquid preparation, container Comes in a convenient dropper bottle—no need to dilute, weigh, or measure out
• Stable at room temperature—keeps it convenient in your range or cell culture area.

See other ReadyProbes™ reagents for cell staining
Learn more about other assays of apoptosis

Cell Imaging Applications
CellEvent™ Caspase-3/7 Green Reagent is a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye that does not fluoresce when not bound to DNA. CellEvent™ Caspase-3/7 Green Reagent is inherently non-fluorescent because the DEVD peptide inhibits dye binding to DNA. Upon activation of caspase-3/7 in apoptotic cells, the DEVD peptide is cleaved and the free dye can bind to DNA, producing bright green fluorescence. When the dye binds to DNA, there is fluorescence emission at 530 nm, which can be observed using a standard FITC filter set.

Usage Recommendations
• In most cases, 2 drops/mL and an incubation time of 30 minutes are sufficient to produce bright nuclear staining of apoptotic cells; however, for some Cell types, conditions, and applications may require optimization. In this case, simply add more or less droplets until the best stain intensity is achieved.
• If necessary, combine CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent with red or far-red nuclear stain for total cell counts
• CellEvent™ Caspase- The maximum excitation wavelength of 3/7 Green dye is 502 nm and the maximum emission wavelength is 530 nm. Detected through standard GFP and FITC filters.