pcDNA5⁄FRT vector is a 5.1 kb expression vector for use with the Flp-In™ System. This vector contains: 1. CMV promoter for high-level expression in mammalian cells; 2. Ten unique restriction sites for easy cloning; 3. Flp recombination target (FRT) site for Integrate the vector into Flp-In™ host cell lines mediated by FLP recombinase, and 4. Hygromycin resistance gene for selection of stable cell lines.