RecoverAll™ Multi-Sample RNA/DNA Workflow is a complete workflow package for extracting samples from the same formaldehyde or poly RNA and DNA were sequentially extracted from formaldehyde-fixed paraffin-embedded (FFPE) tissue samples. Recovered RNA and DNA can be used directly in downstream applications such as quantitative real-time RT-PCR and targeted sequencing using Oncomine™ Cancer Research Plates. This workflow package includes three RecoverAll™ Total Nucleic Acid Isolation Kits, elution tubes, and nuclease-free water.

Features include:
• Recover RNA and DNA from the same FFPE slice into different eluates
• Rely on proven Ambion™ RecoverAll technology
• Every reaction Processes 4 20-μm sections
• Provides enough reagents for 60 RNA and 60 DNA isolations (120 total)

Special workflow provides more information from less sample input
FFPE samples are very expensive, which often requires choosing one extraction method over another to preserve the sample. Use the RecoverAll multi-sample RNA/DNA workflow to recover RNA and DNA from the same FFPE tissue sample in one workflow. There is no need to choose which nucleic acid to recover from a specific FFPE section.

First, the loaded FFPE slides or tissue sections are deparaffinized and digested with protease. The sample is then loaded onto the filter; the DNA remains bound to the filter as the RNA flows through. To recover RNA, the flow-through is applied to a new filter element, treated with DNase, and eluted from the filter column. To recover DNA, the DNA bound to the filter element is first treated with RNase A and then eluted from the filter column.

Related products
Ambion RecoverAll Total Nucleic Acid Isolation Kit< /a>