The need to improve the ability to identify low-level biomarkers has forced the analytical industry to face extremely high levels of analyte and sample complexity, making quantitation a challenging endeavor.

Since these proteins are often present at low levels in complex biological matrices, an immunoaffinity capture step is often used to purify the sample and increase sensitivity. This step is often followed by protein digestion. Current processes are often time-consuming, multi-step, and prone to non-reproducibility.

These IA kits offer a process that is:

• Fast
• Simple
• Highly reproducible
• Sensitive

In the SMART Digest IA kit, the immunocapture reagent and temperature-activated, thermostable trypsin are co-immobilized on the resin. Following enrichment, digestion is accelerated by high temperature activation of the enzyme under protein denaturing conditions, resulting in a workflow that takes hours instead of overnight.