Recombinant human laminin 521 (rhLaminin-521) is a recombinant human protein used to generate human pluripotent stem cells ( Feeder-free culture of PSCs provides a well-defined surface. It has been confirmed that when used together with Essential 8™ Medium, even in the absence of small molecule inhibitors, rhLaminin- 521 may also promote cell survival under stress conditions. Studies have shown that rhLaminin-521 is able to support PSC growth for >20 passages without any evidence of karyotypic abnormalities and maintain the ability of PSCs to differentiate into all three germline lines. When used in combination with Essential 8 Medium, rhLaminin-521 also provides ideal reprogramming efficiency for human cells during the transition from feeder-dependent to feeder-free culture and optimal survival for human pluripotent stem cells. For a kit that combines media and substrate, see Essential 8™ Adaptation Kit.

rhLaminin-521 offers the following benefits:
•Robust – transitioning cells between feeder-dependent and feeder-free culture systems requires minimal effort
>• Efficient—easily reprogram human fibroblasts into pluripotent stem cells
• Physiologically relevant—can expand cells with minimal differentiation
• Scalable—passage stem cell lines as single cells , does not affect genetic stability

Trouble-free transition
When used with Essential 8 Medium, rhLaminin-521 provides a robust culture medium that is independent from feeder layers Supports adhesion and expansion of pluripotent stem cells during culture system transition.

Efficient reprogramming
rhLaminin-521 has been shown to increase the number of colonies obtained during reprogramming of human fibroblasts, which is important for the construction of in vitro disease models .

Reliable expansion
rhLaminin-521 is expressed in blastocysts, thus providing an environment that mimics the human stem cell niche.

Easy to scale up
rhLaminin-521 promotes stable maintenance of pluripotent stem cells as single cells in the absence of ROCK inhibitors.