PureLink Expi Endotoxin-Free Macro Plasmid Purification Kit uses an enhanced anion exchange membrane to quickly and easily isolate endotoxin-free plasmids DNA. Purify up to 15 mg of high-quality plasmid DNA in as little as 2 hours for advanced transfection applications such as sensitive cell line transfection or genome engineering.

PureLink Expi Endotoxin-Free Macro Plasmid Purification Kit benefits include:
•Simple and fast protocol—purification typically takes 2 hours
•High yield—isolates up to 15 mg of high-quality plasmid DNA
• Endotoxin-free plasmid—less than 0.1 EU/µg, ideal for sensitive applications
• High purity—ultra-low levels of protein, RNA, and genomic DNA contamination
• Compatibility—suitable for use with complexes ExpiCHO and Expi293 expression systems for use together
• Endotoxin-free components—endotoxin-free elution buffer, water, and plasmid storage tubes

Simple and fast protocol
with Unlike other anion-exchange endotoxin-free plasmid purification systems, the PureLink Expi Endotoxin-Free Macro Plasmid Purification Kit utilizes centrifugation or vacuum-assisted filtration for lysate purification and subsequent plasmid DNA purification. There is no need to wait for the gravity flow column, the entire purification process usually takes 2 hours (including the final precipitation step).

Endotoxin-Free Plasmids
Purified endotoxin-free (less than 0.1 EU/µg) plasmids are ideal for sensitive applications (e.g. transfection of primary cells, genome engineering, in vivo gene Ideal for therapeutic or plasmid vaccine research).

Compatible with ExpiCHO and Expi293 Expression Systems
Plasmids purified using the PureLink Expi Endotoxin-Free Macro Plasmid Purification Kit are fully compatible with the Gibco ExpiCHO and Expi293 Mammalian Transient Expression Systems .

High Quality, Endotoxin-Free Components
The kit is of high quality and features additional endotoxin-free elution buffer, water and plasmid storage tubes for peace of mind use.