Dynabeads™ CD14 are superparamagnetic microbeads covalently coupled to anti-human CD14 antibodies that can be obtained directly from whole blood, Easily isolate or remove human CD14⁺ cells from any sample including bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads™ CD14:

• Rapid and efficient isolation of CD14⁺ cells from any sample—no columns required
• Positive isolation of CD14⁺ cells have high purity, yield and survival rate
• CD14⁺ cells can be quickly and efficiently removed from any sample

About Information about Dynabeads™ CD14
Dynabeads™ CD14 are uniformly sized superparamagnetic microbeads (diameter 4.5 µm) coated with primary monoclonal antibodies against the CD14 membrane mainly expressed on human monocytes Antigens are specific. Due to the size of the beads, Dynabeads™ CD14 can easily and efficiently separate or remove cells from viscous samples such as whole blood and bone marrow in approximately 30 minutes. Dynabeads™ CD14 also enables the efficient recovery of highly pure and viable cells for downstream molecular studies; for example, cells are lysed but remain attached to the beads and further purified nucleic acids or proteins. Note that intact cells will not be released from these beads, so if you want to isolate CD14⁺ cells for cell-based applications or you need to examine your samples with flow cytometry, Please view our company's Full supply of human monocyte products.

Magnetic bead-based separation technology for easy operation
Add Dynabeads™ CD14 to the sample under continuous mixing to optimize the binding of Dynabeads™ to target cells . Place the sample on the magnetic stand and separate the bead-bound target molecules from the rest of the sample in just 1–2 minutes. To remove, transfer the supernatant to a new tube for further study and discard the bead-bound cells. When performing positive isolations for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to obtain ideal purity. Cells can be lysed but still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples include whole blood, buffy coat, bone marrow, PBMC, or tissue digest products.

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