PureLink™ HiPure BAC Buffer Kit provides additional buffers and reagents for use with the PureLink™ HiPure Plasmid Kit Perform BAC (Bacterial Artificial Chromosome) DNA isolation. PureLink™ HiPure Plasmid Kit utilizes a proprietary anion exchange resin to purify high-quality BAC DNA from E. coli cultures. BAC DNA requires the use of larger bacterial cultures to obtain reasonable DNA yields. Additional buffers and reagents required for processing larger bacterial cultures are provided in this buffer kit. Using 100 mL of culture, it is expected to purify ∼40 µg of BAC DNA. PureLink™ HiPure BAC Buffer Kit provides sufficient reagents for 5 BAC Maxipreps, 25 BAC MidiPreps, or 100 BAC Minipreps.