Thermo Scientific EZ-Link Sulfo-NHS-LC-LC-Biotin targets antibodies, proteins and any other primary amine-containing Simple and efficient biotin labeling of macromolecules. Specific labeling of cell surface proteins is another common application for these unique water-soluble, membrane-impermeable reagents.

Thermo Scientific No-Weigh products are specialty reagents available in pre-portioned formats. Pre-weighed packaging eliminates repeated opening and closing of bottles, thereby preventing loss of reagent reactivity and contamination over time. With this format, a fresh vial of reagent can be used at a time, eliminating the hassle of weighing small amounts of reagent and reducing concerns about reagent stability.

EZ-Link Sulfo-NHS-LC-LC-Biotin Features:

•Protein Labeling—Biotinylated antibodies aid in fixation, purification or Detection (using strepavidin resin or probes)
• Cell Surface Labeling—Only whole cell surface proteins are biotinylated because negatively charged reagents do not permeate cell membranes
• Amine reactivity—reacts with primary amines (-NH2) (such as lysine (K) side chain or polypeptide amino terminus)
• Solubility — Compared with ordinary NHS ester compounds, the charged sulfo-NHS group can improve reagent-grade water solubility
• Irreversible — Form a permanent amide bond; the spacer arm is not cleavable
• Double length—The spacer arm (total length added to the target) is 22.4 Angstroms, this extended arm helps minimize steric hindrance for biotin binding

Sulfo-NHS-LC -LC-Biotin is the longest of three very similar EZ-Link reagents that are water-soluble, non-cleavable, and allow simple synthesis of antibodies, proteins, and any other primary amine-containing macromolecules in solution Efficient biotinylation. Specific labeling of cell surface proteins is another common application for these unique water-soluble, membrane-impermeable reagents. Three Sulfo-NHS-ester reagents that differ only in spacer arm length optimize labeling and detection experiments in which steric hindrance of biotin binding is an important factor.

We manufacture biotin reagents to ensure the highest possible level of overall product integrity, consistency and performance for intended research applications.

BiotinN-hydroxysulfosuccinimide (NHS) ester is the more commonly used biotin chemical reagent type. NHS-activated biotin reacts efficiently with primary amine groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (such as antibodies) often have several primary amines that can be targeted for labeling, including the side chain of a lysine (K) residue and the N-terminus of each polypeptide.

BiotinThe various NHS ester reagents vary in length, solubility, cell permeability and cleavability. Non-sulfonated NHS-Biotin is cell permeable but must be dissolved in organic solvents such as DMSO or DMF. Sulfo-NHS biotin (and biotin with a PEGylated spacer) can be dissolved directly in water but is not membrane permeable. Various types containing disulfide bonds can be cleaved using reducing agents, thereby separating the biotin groups from the labeled protein.