Thermo Scientific EZ-Link HPDP-Biotin is a membrane-permeable biotin labeling reagent that can be used with Thiol (-SH) group reaction. The disulfide bond created between the target sulfhydryl molecule and the biotin group can be cleaved by a reducing agent to release the biotin group and regenerate the original unmodified protein (or peptide). When using immobilized avidin, strepavidin, or Thermo Scientific NeutrAvidin proteins, labeling with HPDP-biotin facilitates purification of target molecules for reducing SDS PAGE or mass analysis; captured biotinylated molecules can Efficient elution from the carrier is achieved by cleavage of disulfide bonds with dithiothreitol (DTT) or other reducing agents, rather than attempting to dissociate the high-affinity interaction between avidin and biotin using strong acids or denaturants.

Thermo Scientific No-Weigh products are specialty reagents available in pre-aliquoted formats. Pre-weighed packaging eliminates repeated opening and closing of bottles, thereby preventing loss of reagent reactivity and contamination over time. With this format, a fresh vial of reagent can be used at a time, eliminating the hassle of weighing small amounts of reagent and reducing concerns about reagent stability.

EZ-Link HPDP-Biotin Features:

•Protein Labeling—Biotinylated antibodies or other proteins for protein methods
• < b>Thiool reactivity—reacts with sulfhydryl (-SH) groups such as cysteine (C) side chains
• pyridyldisulfide activation—in PBS The reaction is carried out in an equal buffer at pH 6.5 to 7.5
• Reversibility—can form a disulfide bond, which can be cleaved using DTT or other reducing agents
• Solubility—Must be dissolved in DMSO or DMF and then further diluted in buffer solution
• Medium Length—Spacer arm (total length added to target) is 29.2 Angstroms

HPDP-Biotin is a pyridyl disulfide-biotin compound that binds to labeled protein cysteine and other molecules containing sulfhydryl groups. This reagent specifically reacts with reduced thiols (-SH) in near-neutral buffers to form reversible disulfide bonds. HPDP-Biotin is used in labeling and affinity purification applications that require recovery of the original, unmodified molecule. For example, a protein can be biotinylated, allowed to bind its interactors, then captured onto a streptavidin column and ultimately eluted and recovered by reduction of disulfide bonds with dithiothreitol.

We manufacture biotin reagents to ensure the highest possible level of overall product integrity, consistency and performance for intended research applications.

Biotinylation reagents vary in reactivity, length, solubility, cell permeability, and cleavability. Three types of Sulfhydryl-reactive compounds Available: maleimide, iodoacetyl and pyridyldisulfide. Pyridyl disulfide reagents react specifically with sulfhydryl groups (-SH) in near-neutral buffers to form reversible disulfide bonds.

In proteins, sulfhydryl groups are found where cysteine (C) residues are present. The cystine disulfide bond must be reduced in order to obtain a sulfhydryl group that can be labeled. The disulfide bonds in the hinge region of antibodies can be selectively reduced to form functional half-antibodies that can be labeled.

Applications:
• Retrieve cell surface receptors and cleave biotin from immobilized avidin columns