Labeled calcium indicators are molecules whose fluorescence intensity increases upon binding Ca²⁺ ions. It can be used for a variety of calcium signal transduction studies, including measuring intracellular Ca²⁺, tracking Ca²⁺ entry and release, and analyzing Ca^{2 in living tissues. +} Perform multiphoton excitation imaging. The AM ester form of this calcium indicator can be loaded onto the cells by adding the dissolved indicator directly to the culture dish containing the cultured cells. The fluorescent signal emitted by such cells is usually measured using fluorescence microscopy, fluorescence microplate detection, or flow cytometry.

Calcium Indicator (AM Ester) Specifications:
• Label (Excitation/Emission Wavelength): Calcium Green™ -1 (506/531 nm)
• Binds Ca ^{The fluorescence intensity increases after 2+}: ∼14 times
• After binding Ca²⁺, the fluorescence increases and the wavelength changes slightly

Spectral Properties of Molecular Probes™ Calcium Indicators
These probes are excited by visible light and are less likely to cause photodamage to cells due to the lower energy required for excitation. Commonly used laser instruments, such as confocal laser scanning microscopy, can effectively excite such indicators, and their emission spectra lie in a spectral region that is usually free of interference from cellular autofluorescence and scattering background.

A wider selection of fluorescent calcium indicators
We offer a large selection of Molecular Probes™ calcium indicators that can be used in a variety of experimental protocols, such as dextran forms to reduce leakage and Compartmentalized, BAPTA conjugates are used to detect high-amplitude calcium transients. For more information, see the Molecular Probes™ Handbook Visible light-excited Ca²⁺ fluorescent indicators—Section 19.3.

For UV excited Ca²⁺ indicators, protein-based Ca²⁺ indicators, Ca²⁺ indicators Conjugates of reagents and fluorescent indicators of other metal ions (i.e. Mg²⁺, Zn²⁺), please see Ca²⁺, Mg²⁺, Zn²⁺, and other metal ion indicators—Chapter 19.

For scientific research use only. Not intended for treatment or diagnosis in animals or humans.