pLenti6.2⁄ V5-DEST™ Gateway™ Vector is a Gateway™-adjusted ViraPower™ II lentiviral expression vector, For lentiviral-based target gene expression in dividing and non-dividing mammalian cells. The vector has a CMV promoter and a PGK promoter. The CMV promoter is used to drive the constitutive expression of the target gene, and the PGK promoter is used to drive the long-term and persistent expression of the blasticidin stable selection marker.
Advantages
• Stable expression
• Long-term experiments
• High-throughput screening
• Accurate titers of functional viruses (using Magnaporthe oryzae method)
•Flexible and versatile Gateway™ recombinant cloning technology
Main Features
• Human cytomegalovirus (CMV) immediate early promoter controls the gene of interest High level expression
•PKG promoter for expression of blasticidin selection marker
Kit includes
• pLenti6.2⁄V5-DEST™ Gateway™ Vector Cassette
•One Shot™ Stbl3™ Chemically Competent E. coli (C7373-03)
Related SKU
• 293FT Cell Line (R70007; R70007)
• ViraPower™ Lentiviral Support Kit (K4970-00)
• ViraPower™ Lentiviral Packaging Mix (K4975-00)
• Lipofectamine™ 2000 (11668-019; 11668-027)
Research use only. Not for therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.