Its special chemical composition prevents quenching (photobleaching) of fluorescent dyes during fluorescence microscopy experiments, providing this protection without significant quenching of the initial fluorescence signal. ProLong Gold Antifade Mounting Medium is a curing mounting medium that creates a near-perfect optical pathlength (1.47 RI), ensuring long-term storage of samples. Ready-to-use (store at room temperature and no mixing required)—just drop in sample, cover with slide, and ready to cure and image. ProLong Gold mounting medium is available with or without DAPI nuclear stain. It is not recommended to use ProLong Gold anti-fade mounting medium to mount samples containing fluorescent proteins (such as GFP). To provide excellent antifade protection for fluorescent proteins and fluorescent dyes, ProLong Diamond Antifade Mounting Medium is recommended.

• Avoid dye quenching during imaging
• Ready-to-use liquid, long-term storage after curing
• Sealed Post-chip samples are stable for months
• Preserve fluorescence signal—little or no quenching
• Ideal for Alexa Fluor™ dyes

• Photobleaching protection, no quenching
• Signal is stable during imaging and can be stored for a long time
• No need Acrylic resin can achieve excellent refractive index matching

It is not recommended for sealing samples containing fluorescent proteins (such as GFP).

Cell analysis, cell imaging, immunofluorescence (IF), immunofluorescence counterstaining, mounting and anti-fade