Thermo Scientific HisPur Ni-NTA Resin is a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His tags fusion protein.

HisPur Ni-NTA Resin Features:

•High Capacity—can bind up to 60 mg of 6xHis-tagged protein per ml of resin
• Versatile—Proteins can be purified under native or denaturing conditions
• Compatible—For use with Thermo Scientific cell lysis reagents and a variety of buffer additives
• Cost-effective—One batch of resin can be reused at least five times
• Easy to use—Kit specifications provide pre-prepared buffers
• Flexible —Available in a variety of formats, including bulk resin, spin columns, chromatographic purification columns, and 96-well filter plates

Specially prepared supports contain microbeaded agarose chelated using nitrilotriacetic acid (NTA) Partially derivatized with chelated divalent nickel ions (Ni²⁺). Immobilized Metal Affinity Chromatography (IMAC) resin has excellent binding capacity and performance for purification of recombinant His-tagged proteins.

Expression and purification of recombinant proteins are critical for the study of protein regulation, structure and function. Most recombinant proteins are expressed as fusions of short affinity tags, the most popular being the polyhistidine (6xHis) tag. The method used to purify recombinant His-tagged proteins is immobilized metal affinity chromatography (IMAC), which consists of a chelating resin with nickel or cobalt ions coordinated to histidine side chains.

HisPur Ni-NTA resin effectively purifies high-level overexpressed His-tagged fusion proteins from bacterial lysates such as those obtained using Thermo Scientific B-PER Bacterial Protein Extraction Reagent. The resin performs well in batch coupling and spin column procedures of various capacities. Performance equals or exceeds that of a variety of commonly used nickel-NTA resins from other suppliers. HisPur Nickel-NTA resin is a high-quality, stable and elastic affinity carrier. Various tests confirm that its performance does not degrade after at least five repeated uses. These data show that the resin is highly resistant to structural degradation or nickel ion leaching during normal use.

HisPur Ni-NTA resin and HisPur Cobalt resin are alternative forms of IMAC, the former using nickel and the latter using cobalt as the chelating metal ion responsible for His-tag binding. Nickel-NTA resin has become the most commonly used IMAC resin choice for 6xHis-tagged protein purification due to its four metal binding points on the chelate that enable high protein binding and low metal ion leaching. The cobalt linkage is less robust and therefore more discriminative, allowing for higher purity but often lower yields in His-tagged protein purifications.

Available Forms:
•Resin Slurry—Cross-linked 6% beaded agarose; 10 mL, 100 mL, 500 mL bottles< br>•Spin Columns— 0.2 mL (microcentrifuge), 1 mL, and 3 mL Spin Columns
•Purification Kits— Complete set of all three column sizes Kit
•Chromatography Purification Column—Learn more and see HisPur Chromatography Purification Column data
•96-well centrifuge plate

Related products
HisPur™ Ni-NTA Spin Column, 0.2 mL
HisPur™ Ni-NTA Spin Column, 1 mL
HisPur™ Ni-NTA Spin Column, 3 mL
HisPur™ Ni-NTA chromatography purification column, 1 mL
HisPur™ Ni-NTA Superflow Agarose